高效液相色谱法测定玉米叶片中的酚酸类化合物_苏贻娟.pdf

DOI:10.13597/j.cnki.maize.science.2009.05.041 玉 米科学2009 ，17(5)：166～168 Journal of Maize Sciences 文章编号：1005- 0906(2009)05- 0166- 03 高效液相色谱法测定玉米叶片 中的酚酸类化合物 苏贻娟，冯远娇，罗赐君，王建武 (华南农业大学农学院，广州 510642) 摘 要：以植物中主要具有防御功能的8 种酚酸作为对照，采用高效液相色谱法测得玉米叶片中主要含有香 草酸、咖啡酸、丁香酸及香豆酸4 种酚酸类化合物。选择Hypersil ODS C18 为色谱柱，乙腈- 水体系为流动相，梯度洗 脱，各酚酸实现有效分离。香草酸、咖啡酸、丁香酸和香豆酸4 种酚酸类化合物在0～57.3 g/mL、0～95.1 g/mL、0～ 28.8 g/mL 和0～47.7 g/mL 浓度范围内具有良好的线性，相关系数大于0.999。加标回收率分别为101.2%～115.1%、 88.7%～97.0%、106.5%～112.7%和96.8%～102.7%。在测定浓度范围内，该方法准确、可靠，可用于玉米等植物叶片 中酚酸类化合物的分析测定。 关键词：玉米；酚酸；高效液相色谱法 中图分类号：S513 文献标识码：A Determination of Phenolic Acids on Corn Leaves by High Performance Liquid Chromatography SU Yi- juan, FENG Yuan- jiao, LUO Ci- jun, WANG Jian- wu (College of Agronomy, South China Agricultural University ，Guangzhou 510642, China) Abstract: Used the main eight kinds of phenolic acids with plant- defensive function as a control,and a high per- formance liquid chromatography to detected the leaves of corn that containing vanillic acid, caffeic acid, syringic acid and coumaric acid. Four compounds were successfully separated on a Hypersil ODS C18 analytical column using a mo- bile phase of acetonitrile- water with gradient elution. Four phenolic acids exhibited good linear relationship in the concentrations ranging 0 - 57.3 g/mL, 0 - 95.1 g/mL, 0 - 28.8 g/mL and 0 - 47.7 g/mL, with the correlation coeffi- cient higher than 0.999. Their recoveries were 101.2% - 115.1% ，88.7% - 97.0% ，106.5% - 112.7% and 96.8% - 102.7%, respectively. It could be seen that within the linear range, the method was accurate and reliable. Ther