高效液相色谱法测定青梅花枝叶中绿原酸类化合物.pdf

※分析检测 食品科学 2011, Vol. 32, No. 02 191 高效液相色谱法测定青梅花、枝、叶中 绿原酸类化合物 1 2 石嘉怿 ，张 英 ( 1.南京财经大学食品科学与工程学院，江苏南京 2 10003 ； 2.浙江大学生物系统工程与食品科学学院，浙江杭州 3 10029) 摘 要：目的：建立反相高效液相色谱法同时测定青梅非果部位(花、枝、叶) 中新绿原酸(NCA) 、绿原酸(CA)及 隐绿原酸(CCA)3 种绿原酸异构体的含量。方法：采用高效液相色谱法梯度洗脱，使用Luna C18 色谱柱，以体积 分数2% 乙酸溶液和乙腈为流动相，二极管阵列检测器在波长328nm 处进行检测。结果：该方法线性范围宽，3 种绿原酸异构体的线性范围均为5 ～200 μg/mL ，NCA 、CA 、CCA 的检出限分别为0.2 1、0.16 、0.29 μg/mL ，定 量限分别为0.76 、0.50 、0.98 μg/mL ，测得青梅花、枝、叶的30% 乙醇提取物中3 种绿原酸异构体的总含量分别 为(4 8.2 6 ±0 . 11) 、( 10 .82 ±0 .04 ) 、(2 1.3 3 ±0 .0 8)μg/ g 。结论：该方法简便、快速、准确，青梅花、枝、叶是 优良的绿原酸类化合物天然资源。 关键词：青梅；高效液相色谱；新绿原酸；绿原酸；隐绿原酸 HPLC Determination of Chlorogenic Acids in Flowers, Branches and Leaves of Prunus mume 1 2 SHI Jia-yi ，ZHANG Ying (1. School of Food Science and Engineering, Nanj ing University of Finance and Economics, Nanj ing 2 10003, China ； 2. School of Biosystems Engineering and Food Science, Zhej iang University, Hangzhou 3 10029, China) Abstract ：Three chlorogenic acid isomers, namely, neochlorogenic acid, chlorogenic acid, and cryptochlorogenic acid in the flowers, branches, and leaves of Prunus mume were simultaneously determine by a HPLC method. The samples were separated by a Luna C18 column using 2% acetic acid and acetonitrile as a mixed mobile phase. The detection wavelength was 328 nm. The analytical method displayed a wide linear range of 5 to 200 μg/mL for the three chlorogenic acid isomers. The detection limits were 0.2 1, 0.16 μg/mL and 0.29 μg/mL for neochloroge