脑脊液细胞学检查是一种细胞形态学检验。脑脊液-第三军医大学学报.doc

中枢神经系统感染脑脊液细胞形态特征分析 吴若芬1.2 王妍柏1.2 何学仙1.2. 窦春阳2.3 王振海1.2.3 1.宁夏医科大学总医院脑脊液研究室 750001 2.宁夏颅脑疾病重点实验室 3.宁夏医科大学总医院神经病学中心 摘要 目的：探讨中枢神经系统感染脑脊液中细胞形态的特征改变，测量脑脊液中各类细胞面积、直径的大小，提高脑脊液细胞学分类的准确性，实现在颅内感染疾病的早期诊断。方法：应用细胞玻片离心仪制片、瑞氏-姬姆萨（MGG）染色，细胞显微图像分析系统观察细胞形态。选择“抓图”以688×512像素摄取细胞图像，对脑脊液的各类有核细胞进行细胞面积、直径等测量，分析不同疾病中细胞特征的大小变化。结果：所观察分析的736例的患者分为：病毒性脑膜炎260例，淋巴细胞占(86.51±12.50)%；其中激活淋巴细胞占(17.95±14.09)%。化脓性脑膜炎88例，中性粒细胞占(80.88±10.30)%，可见杆状核细胞和先令指数左移。术后感染150例，中性粒细胞(85.55±7.91)%，严重的胞浆内可见中毒颗粒。新生儿颅内感染230例，单核细胞占(72.33±19.78)%，其中激活单核细胞占(8.38±6.51)%。根据测量得到的特征参数结果进行对比后，发现测量的面积、直径和形态特征对颅内感染病种具有指导性意义。结论：细胞形态学特征的改变是诊断神经系统感染疾病的重要指标。 关键词：脑脊液细胞学；细胞玻片离心仪；细胞显微图像分析系统；细胞测量； Morphology?analysis?of?Cerebrospinal?fluid?cell?and?clinical?significance?in?different?diseasesRuofen?Wu;?Yanbo?Wang;Xuexian?He;Chunyang?Dou;Zhenhai?Wang? Ncurolymph?laboratory,General?Hospital?of?Ningxia?Medical?University?Ningxia?Key?Laboratory?of?Cerebrocranial?Diseases [Key?words]:Cerebrospinal?fluid?cytology;Cerebrospinal?fluid?cytocentrifuge?instrum-ents;Cell?microscopic?image?analysis?system;Cellular?measurements; [Abstract]?Objective:To?investigate?changes?of?morphological?characteristics?of?all?kinds?of?white?blood?cells,measuring?sizes?of?areas?and?diametesr?of?that?in?Cerebrospinal?fluid,significance?in?for?clinical?diagnosis?in?diferent?diseases.That’ll?improve??the?accuracy?of?the?cytological?classification?of?Cerebrospinal?fluid?and?implementate?the?early?diagnosis?in?different?diseases.Methods:?We?dealded?with?samples?to?centrifuge?by??cell?slice?centrifugal?instrument,stain?by?Wright?-?Giemsa?,?observe?all?kinds?of?cells?morphology?by?Cell?microscopic?image?analysis?systemc.Taking?cells?pictures?as?688*512?pixel,which?measured?area?and?diameter?of?all?kinds?of?nucleated?cells?in?cerebrospinal?fluid,?analyzed??the?characteristics?of?the?changes?in?different?diseases. Results:There?were?736?patients?tha