舞茸提取物D-fraction能增强NK细胞的细胞毒性作用.docx

声明：本文摘自Oncology Reports，由希腊知名的科技出版社Spandidos Publications出版发行，创刊于1994年，月刊。该杂志内容覆盖肿瘤领域广泛的基础和应用研究，注重于发表肿瘤发生、转移和流行病学方面的原创性论文和综述。为方便广大读者阅读，我们对论文摘要进行了中文翻译，译文仅供参考，如有不足之处，敬请指正。 -----本文摘自舞茸D-fraction官方网站，转载请注明 【参考译文】 ????????????????????????????? ?Enhancement of cytotocicity of NK cells by D-Fraction,????????????????????????????????????????? ?a polysaccharide from Grifola frondosa????????????????????????????????舞茸提取物D-fraction能增强NK细胞的细胞毒性作用 ??????????????????????????????????????????????????????????????????? 摘? 要 ??? 在先天免疫中，在不受MHC抗原限制的情况下被激活的自然杀伤（NK）细胞能攻击和消灭病原体如细菌、病毒。有报道称被IL-12激活的NK细胞在穿孔蛋白介导的细胞凋亡中能识别和杀死肿瘤细胞。我们曾报道，一种从舞茸中提取的多糖D-fraction能激活巨噬细胞、树突细胞以及T细胞，并能抑制肿瘤生长。尽管如此，舞茸D-fraction 在先天免疫反应中对NK细胞功能的影响并不清楚。在此项试验中，MM-46乳腺癌荷瘤小鼠连续三天腹腔内注射舞茸D-fraction，从而研究其对NK细胞活性和细胞毒性的作用。结果显示D-fraction能显著增强NK细胞对于NK敏感性靶细胞YAC-1细胞的细胞毒性以及其CD223的表达量。D-fraction也能增加巨噬细胞的CD86表达量。另外，与对照组相比，脾细胞培养上清液以及血清中的IL-12量也增加，相应地NK细胞中IL-12受体β1的表达量也增加。这些结果表明D-fraction通过激活巨噬细胞分泌IL-12来增强NK细胞的细胞毒性。关键词：NK细胞，细胞毒性，白介素-12受体 论文原文 ?????????????????????????????????????????? ONCOLOGY REPORTS 13: 497-502, 2005 ??????????????????????????????? ?Enhancement of cytotoxicity of NK cells by D-Fraction,???????????????????????????????????????????? a polysaccharide from Grifola frondosa NORIKO KODAMA1, AKIHIRO ASAKAWA2, AKIO INUI2, YUKI MASUDA and HIROAKI NANBA1 1Department of Microbial Chemistry, Kobe Pharmaceutical University, 4-19-1, Motoyama- kitamachi, Higashinada-ku, Kobe 658-8558; 2Division of Diabetes, Digestive and Kidney Diseases, Department of Clinical Molecular Medicine, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan ??????????????????????????????????? Received August 10, 2004; Accepted October 4, 2004 Abstract.?In innate immunity, activated natural killer (NK) cells attack and damage pathogens such as bacteria and virus without restriction by the MHC antigen. NK cells activated by IL-12 have been reported to recognize and kill tumor cells in perforin-mediated apoptosis. We have re