胆汁致胃黏膜损伤中瘦素环氧合酶-2及p27的表达.doc

KKME---专业医学搜索引擎/ 胆汁致胃黏膜损伤中瘦素、环氧合酶-2及p27的表达 作者：宋银雪，龚均 作者单位：西安交通大学医学院第二附属医院消化科，陕西西安 710004 【摘要】 　　目的 通过大鼠实验模型观察瘦素(leptin)、环氧合酶-2(COX-2)及p27在胆汁所致胃黏膜损伤中的作用。方法 SD大鼠分成4组：十二指肠胃反流(duodenogastric reflux, DGR)组、DGR+胆管结扎(bile duct ligation, BDL)组、胆管结扎组(BDL)、假手术组(control, C)组。术后12周处死大鼠，观察胃黏膜损害及病理组织学改变，计算DIXON积分；免疫组化(SP)法检测各组大鼠胃黏膜瘦素、COX-2及p27表达。结果 DIXON积分DGR组、DGR+BDL组较BDL组及假手术组积分均升高(P0.05)，DGR组较DGR+BDL组DIXON积分升高(P0.05)；DGR组、DGR+BDL组与BDL组及假手术组比较大鼠胃黏膜瘦素、COX-2高表达(P0.05)，p27低表达(P0.05)，DGR组比DGR+BDL组高表达尤为明显(P0.05)。DIXON积分与瘦素、COX-2表达呈正相关，与p27表达呈负相关。结论 胆汁反流致胃黏膜损伤过程中，瘦素、COX-2及p27蛋白与病变的修复过程有关。 【关键词】 胆汁 胃黏膜 瘦素 COX-2 p27 　　Expressions of leptin, COX-2 and p27 in bile-induced gastric mucosa injury 　　Song Yinxue, Gong Jun 　　(Department of Gastroenterology, the Second Affiliated Hospital,Medical School of Xian Jiaotong University, Xian 710004, China) 　　ABSTRACT: Objective To explore the role of leptin, COX-2 and p27 in bile-induced gastric mucosal injury in rats. Methods SD rats were divided into 4 groups: DGR (duodenogastric reflux) group, DGR+BDL (ligation of bile duct) group, BDL group and control group. The pathological changes of gastric mucosa and tight junction were observed, DIXON scores were warked out after 3 months. Immunohistochemistry was used to detect the expression of leptin, COX-2 and p27 in gastric mucosa with different lesions. Results The DIXON scores in DGR and DGR+BDL group were higher than those in BDL group and control group (P0.05). In addition, DIXON scores were higher in DGR group than in DGR+BDL group (P0.05). The expressions of leptin and COX-2 increased in DGR group and DGR+BDL group than in BDL group and control group (P0.05), while p27 decreasd (P0.05). DGR group was more notable than DGR+BDL group (P0.05). The expression of leptin and COX-2 was positively correlated with DIXON integral, but negatively related to p27. Conclusion Leptin, COX-2 and p27 are all concerned with renovating pathological changes in bile-induced gastric