福州宦溪野生蕉(胁s口spp．，ABgroup)2个.pdfVIP

热带作物学报2013，34(1)：046—053 垦垦!里旦!竺!!竺竺兰!垡!垫旦!竺璺!垦翌旦! 福州宦溪野生蕉(胁s口spp．，AB group)2个 P舛G成员的克隆及生物信息学分析 刘炜嫡．赖钟雄4 福建农林大学园艺植物生物工程研究所．福建福州 350002 摘 要 植物光系统I反应中心亚基V(photo即stem I reaction center sLlbunit V，简称PsAG或PS I—G)是光合 系统I的主要组件。具有维持PS I复合体稳定性的重要作用，并与抗盐密切相关。本研究以福州宦溪野生蕉 (M琊G spp．AB group)叶片为材料，采用同源克隆的方法，首次分离到PSAG基因的2个成员：嬲AGZ、 Ps4G2(GenBank登录号分别为Jx317082、JX317083)，分别为800、827 bp，分别编码150、160个氨基酸； 憨AGj、PsAG2的基因组序列分析表明2个成员均没有内含子。生物信息学分析表明：PSAGl、PsAG2具有PS I的X亚基超家族(photosystem I reaction center subunitXpsaK)保守结构域，是不具有信号肽的跨膜蛋白，具有 亲水性；PSAGl、PSAG2均有4个位点发生磷酸化。宦溪野生蕉黝G在进化过程中形成了特殊的结构特征． 即P趴GJ和愿dG2没有内含子，并且在不同物种间保守区具有高度的一致性．为保持殿谢G功能的稳定性提供 了重要保证。 关键词 宦溪野生蕉(肘琊o spp．AB group)；只姒G；基因克隆；内含子；生物信息学 中图分类号S668．1 文献标识码A Cloning and Bioinfomatics Analysis of Two Members of p苎汹G in the Wild Banana(2I缸s口spp．，AB Group) from Huanxi Town in Fuzhou LIU Weihua，LAI Zhon舒iong jm斑me《H积kHz泐矗Bimech黯0lo戳脚im A薛c ul￡uM∞d F。『es叼UR协ersi％阮b地％洫350002．Cht眦 Abstract PhotosYstem I I℃action center subunit V(I毪；AG or PSI—G)is one of the main components of photosvstem I， and it is important for the stability of the PS I complex，and closely related with salt resistance．In this report，the leaves of the wild banana(7盯嬲口spp．，AB Gmu讲f南m Huanxi Town were used as the mate“a18 for cloning f)5A G genes by the homologous cloning method， and the two members of只SA G were firstly isoIated and named as f姒GJ and PSAG2(the accessiofl numbeI_s were JX317082 and Ⅸ317083 in GenBank，respectivelv)． The sequences of the full—lerlgth cDNA of．尸：SA GJ and．P：SA G2 weIe 800 bp and 827 bp，encoding 150 and 160 amino acids，respectively．The genomic DNA sequencing analysis of．尸：SA G，and．P：SH G2 indicated that there weI℃ no introns in the two meInbers． BioinfonIlatics analysis showed that both of the two members of PSAG were with the supe卜family conservative domain of photosystem I reaction center subunit X(psaK)，and t}ley were hydrophilic and 奶thout the signal peptide， belonging to the transmeHlbrane protein． Both PSAGl and PSAG2 had four phosphoIylation sit