骨髓间充质干细胞对前列腺癌细胞生物学行为的影响.doc

骨髓间充质干细胞对前列腺癌细胞生物学行为的影响 刘建军，段小雨(河南省人民医院泌尿外科，河南省郑州市 450000) 引用本文：刘建军，段小雨. 骨髓间充质干细胞对前列腺癌细胞生物学行为的影响[J].中国组织工程研究，2016，20(14): 2046-2051. DOI: 10.3969/j.issn.2095-4344.2016.14.010 ORCID: 0000-0001-7905-7533(刘建军) 文章快速阅读： 文题释义： 前列腺癌DU145细Effects of bone marrow mesenchymal stem cells on the biological behavior of prostate cancer cells Liu Jian-jun, Duan Xiao-yu (Department of Urinary Surgery, Henan Provincial People’s Hospital, Zhengzhou 450000, Henan Province, China) Abstract BACKGROUND: Bone marrow mesenchymal stem cells have a close relationship with tumor occurrence and development, which are not only involved in tumor recruitment but impact the biological behavior of tumor cells. OBJECTIVE: To investigate the effect of bone marrow mesenchymal stem cells on the biological behavior prostate cancer cell line. METHODS: Human bone marrow mesenchymal stem cells from healthy adults were collected and cultured in vitro. Cell morphology and cell markers were observed. Using the Transwell technique, human dermal fibroblasts HDF-a were co-cultured with human prostate carcinoma DU145 cells in positive control group, wild-type prostate carcinoma DU145 cells cultured alone in negative control group, and bone marrow mesenchymal stem cells co-cultured with human prostate carcinoma DU145 cells in experimental group. Flow cytometry assay was used to detect the cell cycle of prostate carcinoma DU145 cells, soft agar colony formation assay performed to detect cell proliferative ability, and crystal violet staining used to observe DU145 cell migration at 3 days after culture. RESULTS AND CONCLUSION: Compared with the negative control group, cell proportion at G2/M+S phase, number of transmembrane cells and colony counting were significantly decreased in the experimental group. These findings indicate that bone marrow mesenchymal stem cells may inhibit the proliferation and invasion of prostate carcinoma DU145 cells by shortening the G2/M+S phase, the