enzymesanddetergents-winona（1页）.doc

Enzymes and Detergents Today’s lab will focus on enzymes in detergents. These enzymes are purposefully put into our cleaning solutions in order to help break down organic matter such as dirt or food. Some common enzymes include: Enzyme Function Protease Digests protein-based stains Amylase Digests starch-based stains Lipase Digests fat or oil-based stains Guardzyme (Peroxidase) Inhibits dye transfer Carezyme Removes fuzz build up on cotton We will look at different detergents with and without enzymes (and our unknown) to determine if the presence of enzymes in detergent actually breaks down (degrades) proteins better than detergents without enzymes. We will also test to see if the concentration of enzymes has any effect on protein digestion and if pH and temperature effect enzyme activity. All of these activities will be done if test tubes of gelatin (our protein source). After setting up all your test tubes, the reaction needs about 24 hours to react. Therefore, at least one person per group needs to come back to lab the next day and measure their results. Work in groups of four and be meticulous about labeling all your test tubes. Make sure you all understand how we are going to measure the tubes (be able to identify millimeters as opposed to centimeters on your rulers). Crime Scene Implications: There was detergent left at the scene of the crime when someone apparently tried to clean up a blood stain. We are going to attempt to determine if this detergent contained enzymes or not, and then we will send our sample to chemistry for further analysis. Day 1 Part 1: A Survey of Various Laundry Detergents: Enzymes vs. No Enzymes 1. Each group will obtain 6 non-labeled test tubes containing gelatin. Label the test tubes 1-6 and your initials (or group name). Place the test tubes in a test tube rack. 2. Take the following five detergents to be tested; Ward’s Detergent with Enzymes, Ward’s Detergent without enzymes, Tide with Blea