Cloning and molecular analysis of structural genes involved in anthocyanin.doc

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Cloning and molecular analysis of structural genes involved in anthocyanin.doc

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Cloning and molecular analysis of structural genes involved in anthocyanin 导读：就爱阅读网友为您分享以下“Cloning and molecular analysis of structural genes involved in anthocyanin”的资讯，希望对您有所帮助，感谢您对92的支持! PlantMolecularBiology35:915–927,1997. c1997KluwerAcademicPublishers.PrintedinBelgium. 915 Cloningandmolecularanalysisofstructuralgenesinvolvedinanthocyaninbiosynthesisandexpressedinaforma-speci?cmannerinPerillafrutescens ZhizhongGong1,MamiYamazaki1,MitsuyoSugiyama1,YoshikazuTanaka2andKazukiSaito1 FacultyofPharmaceuticalSciences,LaboratoryofMolecularBiologyandBiotechnology,ResearchCenterofMedicinalResources,ChibaUniversity,Yayoi-cho1-33,Inage-ku,Chiba263,Japan(authorfor correspondence);2InstituteforFundamentalResearch,SuntoryLtd.,1-1-1,Wakayama-dai,Shimamoto,Mishima,Osaka618,Japan 1 Received13May1997;acceptedinrevisedform18August1997 Keywords:anthocyanin,cDNAcloning,forma-speci?cexpression,genomicorganization,lightinduction,PerillafrutescensAbstract TwocultivarsofPerillafrutescens,redandgreenformas,areknowntodifferinanthocyaninaccumulationinleavesandstems.cDNAclonesencodingtheenzymesinvolvedinanthocyaninbiosynthesis,chalconesynthase(CHS),?avanone3-hydroylase(F3H),dihydro?avonol4-reductase(DFR),andUDPglucose:?avonoid3-O-glucosyltransferase(3GT),wereisolatedfromcDNAlibrariesderivedfromtheleavesofaredformaofP.frutescensbyscreeningwithpartialfragmen