论文翻译14.6.5.doc

The apoptosis induction effect of Oligochitosan on human hepatocyte and hepatoma cells Abstract. Chitosan oligosaccharide (cso), the degradation products of chitin, are confirmed having a wide range of physiological functions and biological activity. In this study, we explored the apoptosis induction effect of COS on liver cancer cells. Our data indicated that with 0.5-2.5 mg/ml oligochitosan effect 24 h, there was a dose-dependent effect, and the inhibition of hepatoma cells was significantly greater than the liver cells. On hepatoma cells and liver cells the IC50 was 2.5 mg/ml, and 3.0-4.0 mg/ml. Treated with 2.5 mg/ml COS for 24 h, the apoptosis of hepatoma cells increased to 76.04%, and the Bcl-2 expression of hepatoma cells reduced, while Caspase-3 increased, but it did not affect the two proteins of hepatocytes. Our results will provide an experimental basis for the clinical development of new toxic side effects of anti-liver cancer drug. Keywords: Oligochitosan, Chang Liver, SMMC-7721, Apoptosis. Introduction Hepatocellular carcinoma is a common malignancy and its incidence is rising recently in the developing world. More than 700,000 new cases are diagnosed throughout the world and more than 600,000 deaths are due to hepatocellular carcinoma each year (1). Hepatocellular carcinoma becomes the seventh most common cancer worldwide, and the third leading cause of cancer-related deaths (2). Convertional chemotherapy is not efficient for liver cancer patients, because of drug resistance the toxic side effects (3). Therefore it is urgent to find a novel therapeutic drug. Oligochitosan, obtained by hydrolysis or degradation of chitin (3), has 3-10 saccharide (N-acetyl-glucosamine or glucosamine) residues (4). Study has been indicated that Oligochitosan has a wide range of biological activities. In particular, Oligochitosan have been reported to have the health benefits such as immunity regulation, anti-tumor, anti-dia