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財團法人彰化基督教醫院 研究計畫經費補助申請書 計畫名稱：Nandrolone and Vivonex Influences Free Radical Responses of Bacterial Peritonitis in Mice 執行期限：自92年1月1日 至92年12月31日 計畫主持人： 林吉吉 所屬單位： 外科部 計畫聯絡人： 林吉吉 聯絡電話： CCH Beeper 276271 本計畫經費預算大綱：（細節另頁列表，並說明估算方法與用途） 項 目 金 額 使用說明摘要 耗材 NTD 266,436.9 實驗所使用的器具、消耗品、試藥 ，細節如另頁列表 人事 NTD 120,000 補助研究助理、飼養動物人工費用、 合作實驗室儀器維修人員費用，每月NTD 10,000 共一年 其他 NTD 10,000 細節另頁列表外，實驗進行所需的花費 Total NTD 396436.9元整 審查核示：  Ｉ、計畫摘要 Many patients with large bowel penetrating injury suffered from E. Coli bacterial peritonitis. Nandrolone have the effect to improve these patients. This proposal focuses on the free radical, cytokine, insulin-like growth factor-1 receptor(IGF-1R) mRNA, insulin-like-binding-protein-3 (IGFBP3) mRNA interaction by E.Coli bacterial peritonitis mice model with nandrolone and Vivonex. E. Coli bacterial peritonitis resulted in sepsis. In peritoneal cavity the number of E. Coli and local concentration of lipopolysaccharide increased. Gu et al reported that will influence the Kupffer cells of liver and result in more free radical and cytokine change. Increasing free radicals cause many syndromes in sepsis including apoptosis, ARDS , multiple organ failure, and mortality. McCord reported free radicals influence intracellular kinases and DNA transcription. However, IGF-1R with tyrosine kinase and was relevant to apoptosis. IGF-1R may help cells resist the injury of free radicals from apoptosis by tyrosine kinase mechanism. In contrast, Renehan reported IGFBP3 might help apoptosis. Cytokine cascade causes septic symptoms and may be related to the free radical, IGF-1R, IGFBP3 mechanism. However, the mechanism how free radicals, cytokines, IGF-1R, IGFBP3 influenced the apoptosis and survival rate remained unclear. Chao et al reported phosphatidylcholine hydroperoxides (PCOOH) and malondialdehyde (MDA) could be markers of free radicals. In this study, we will establish a free radical model by counting the amount of PCOOH and MDA to measure