金属元素的活性探究.docVIP

PAPER /metallomics | Metallomics  Metallic elements in exhaled breath condensate and serum of patients with exacerbation of chronic obstructive pulmonary disease Massimo Corradi,aOlga Acampa,bMatteo Goldoni,abRoberta Andreoli,ab Donald Milton,cSusan R. Sama,cdRichard Rosiello,dGiuseppe de Palma,e Pietro Apostolieand Antonio Mutti*a Received 15th April 2009, Accepted 28th May 2009 First published as an Advance Article on the web 18th June 2009 DOI: 10.1039/b907635b Biomarkers in exacerbated chronic obstructive pulmonary disease may be useful in aiding diagnosis, de?ning speci?c phenotypes of disease, monitoring the disease and evaluating the e?ects of drugs. The aim of this study was the characterization of metallic elements in exhaled breath condensate and serum as novel biomarkers of exposure and susceptibility in exacerbated chronic obstructive pulmonary disease using reference analytical techniques. C-Reactive protein and procalcitonin were assessed as previously validated diagnostic and prognostic biomarkers which have been associated with disease exacerbation, thus useful as a basis of comparison with metal levels. Exhaled breath condensate and serum were obtained in 28 patients at the beginning of an episode of disease exacerbation and when they recovered. Trace elements and toxic metals were measured by inductively coupled plasma-mass spectrometry. Serum biomarkers were measured by immunoassay. Exhaled manganese and magnesium levels were in?uenced by exacerbation of chronic obstructive pulmonary disease, an increase in their concentrations—respectively by 20 and 50%—being observed at exacerbation in comparison with values obtained at recovery; serum elemental composition was not modi?ed by exacerbation; serum levels of C-reactive protein and procalcitonin at exacerbation were higher than values at recovery. In outpatients who experienced a mild–moderate chronic obstructive pulmonary disease exacerbation, manganese and magnesium levels in exhaled breath cond