哺乳动物胚胎冷冻保存的研究进展.pdfVIP

, Journal of Anhui Agri.S i. 2007, 35( 34) : 11107- 11109 陈玉敏 俞洁 1 2 3 王军 , 孙博 , 吕文发 ( 1. , 121001; 2. , 121000; 3. , 130118) [ 目的] 介绍哺乳动物胚胎冷冻保存技术的最新研究进展[ 方法] 冷冻原理防冻剂种类冷冻方法解冻方法等方面综述了哺 乳动物胚胎冷冻保存技术的最新研究进展[ 结果] 胚胎冷冻保存的原理是阻止冷冻解冻过程中细胞内形成致死冰晶; 防冻剂的种类 大致分为低分子量渗透性防冻剂低分子量非渗透性防冻剂大分子物质生物活性物质; 胚胎冷冻方法主要有常规慢速冷冻法玻璃化 冷冻法和快速冷冻法; 解冻方法主要介绍了快速解冻[ 结论] 胚胎冷冻保存技术的应用愈加广泛, 将对胚胎生物工程和其他生物技术 产生推动作用 胚胎; 冷冻保存; 防冻剂; 冷冻; 解冻 Q 954. 4 A 0517- 6611( 2007) 34- 11107- 03 Recent Advances in Cryopreservation of Mammalian Embryos WANG Jun et al ( College of Animal S ien e and Te hnology , Liaoning Medi al University, Jinzhou, Liaoning 121001) Abstract The paper has introdu ed the re ent advan es in ryopreservation of mammalian embryos during the past ten years. This paper has reviewed the latest information on ryopreservation of mammalian embryos, in luding the prin iple of ryopreservation, sorts of ryoprote tant, and method for freezing and thawing. Embryo ryopreservation te hniques mainly in lude onventional slow freezing method, vitrified ryopreservation method and rapid freezing method. The paper has mainly introdu ed the rapid thawingmethods. The broader appli ation of embryo ryopreservation te hniques will play a promotion role in embryo biologi al engineering and other biote hnology. Key words Embryo; Cryopreservation; Cryoprote tant; Freezing; Thawing , - 196 , 2 2. 1 1, 2 , DMSO2, 3 , , 1972 , , Whittingham , [ 1] , , , , , 1 4 , 80% DMSO, , , , 2000 Emiliani [2] , , Valdez , , , , , [ 3] - 10