黄芩苷抗呼吸道合胞病毒感染作用的体外研究.docVIP

黄芩苷抗呼吸道合胞病毒感染作用的体外研究 　　作者：张丽亚 董 琳 陈小芳 王 平 作者单位：温州医学院附属育英儿童医院呼吸科 　　【摘要】 目的 探讨黄芩苷(baicalin)的体外抗呼吸道合胞病毒(RSV)感染作用及最佳浓度。方法 应用微量细胞培养技术，观察RSV感染人喉癌(Hep-2)细胞病变效应(CPE)。观察不同浓度黄芩苷对人肺癌腺上皮(A549)细胞的CPE及RSV感染后CPE的抑制作用。应用四甲基偶氮唑蓝(MTT)比色法检测黄芩苷对A549细胞的毒性作用和RSV感染后细胞存活率的影响。结果 黄芩苷浓度≤0.156mg/ml时，A549细胞未显示明显CPE，MTT比色法结果与CPE观察结果相符;浓度在0.078～1.25mg/ml时，RSV感染的A549细胞仅出现轻微CPE，0.625mg/ml时对CPE有最好的保护作用;治疗指数(TI)为19.23;MTT比色法也显示以上浓度范围黄芩苷组的细胞存活率明显高于RSV感染组，差异有统计学意义(P均0.05)，其中以0.3125 mg/ml组的细胞存活率最高，达82.09%。结论 黄芩苷具有体外抗RSV感染作用，有效浓度为0.078～1.25mg/ml，最佳作用浓度为0.156mg/ml。 　　【关键词】 黄芩苷 呼吸道合胞病毒 抗感染 体外实验 　　【Abstract】 Objectives To search the effect of baicalin for infection of respiratory syncytial virus(RSV)and the best effective concentration. Methods Micro-well cell cultures was adopted，and cytopathogenic effect (CPE) of HEp-2 cells induced by RSV infection were observed through inverted phase-contrast microscopically. The CPE of A549 cells induced by baicalin of different concentration and the effects of baicalin on change of CPE of A549 cells induced by RSV infection were observed through inverted phase-contrast microscopically, too. Methyl thiazolyl tetrazolium (MTT) assay was used to detect cytotoxicity of baicalin on normal A549 cells and protective effects of it on RSV infected A549 cells. Results When the concentration of baicalin was equal or less than 0.156mg/ml, there were no significant CPE in the baicalin group and it has the same result when MTT assay was used;When the concentration extent of baicalin on RSV infected A549 cells were 0.078-1.25mg/ml , there were less CPE and higher survival rate than RSV infected but no baicalin interfered group,and it has the best protection effect at concetration of 0.625mg/ml; the treatment index(TI)of baicalin was 19.23; there has significiant differences between the two groups when MTT assay was used (P0.05)，and it has the max survival rate to 82.09% when the concentration was 0.3125 mg/ml. Conclusions Baicalin can counteract the infection of RSV in