斜带石斑鱼alpha型与rho型GST基因cDNA全序列的克隆与分析.pdfVIP

2008 3 Vol.3, 2008 3 , 250−255 Asian Journal of Ecotoxicology No.3, 250−255 斜带石斑鱼 alpha 型与 rho 型 GST 基因 cDNA 全序列 的克隆与分析 * 胡永乐，梁旭方 ，林群，李光照，李观贵，王琳 暨南大学生命科学技术学院，广州 510632 摘要II S-(Glutathione S-transferase, GST) (Epinephelus coioides)RT-PCR RACE alpha-GST (GSTA)rho-GST (GSTR)cDNA . GSTA cDNA 1008bp223 GSTR cDNA 1002bp225 . GSTA GSTR GST . 关键词S- 文章编号1673-5897(2008)3-250-06 中图分类号Q78 文献标识码A Cloning and Sequence Analysis of the Alpha and Rho-Class GST Genes in Orange-Spotted Grouper (Epinephelus coioides) * HU Yong-le, LIANG Xu-fang , LIN Qun, LI Guang-zhao, LI Guan-gui, WANG Lin College of Life Science and Technology, Jinan University, Guangzhou 510632 Received 26 December 2007 accepted 5 March 2008 Abstract: To research the molecular mechanism of phase II detoxifizyme (glutathione S-transferases, GST) in the metabolism of toxics in marine fish, we cloned the full-length cDNA sequences of alpha-GST (GSTA) and rho-class GST (GSTR) genes from the liver of orange-spotted grouper (Epinephelus coioides) by RT-PCR and RACE methods. The sequence analysis showed that the complete GST cDNA sequence of GSTA of orange-spotted grouper was 1008bp in length, containing a coding region of 672bp (encoding 223 amino acids). And the complete cDNA sequence of GSTR was 1002bp in length, containing a coding region of 678bp (encoding 225 amino acids). Results of the phylogenetic analysis revealed that the deduced amino acid sequence of the orange-spotted grouper was very similar to the GSTA of other fishes, such as common carp and zebrafish.