乳腺癌术后胸膜转移性胸水TIL的细胞毒活性的研究.pdfVIP

乳腺癌术后胸膜转移性胸水 TIL 的细胞毒活性研究 213003 常州 苏州大学附属第三医院肿瘤科 吴昌平 蒋敬庭 邓海峰 孙文辉 吴爱珍 陈国瑾 摘要 目的 探讨乳腺癌术后胸膜转移性胸水TIL的细胞毒活性 方法 本室 采用生物技术 提纯乳腺癌胸膜转移的胸水中的肿瘤浸润淋巴细胞和相应的肿 瘤细胞 经过一定时间诱导培养后 在7天 14天 21天和28天分别检测 TIL对自身肿瘤细胞和K5 62 细胞的杀伤活性 结果 该TIL对自身肿瘤细胞 和K5 62 细胞的杀伤活性无显著性差异 三周时细胞毒活性最强 结论 TIL 诱导培养21天时具有较强的生物活性 此时进行临床应用可取得最佳治疗效 果 关键词 乳腺癌 胸腔积液 恶性 淋巴细胞 肿瘤浸润 杀伤细胞活性 A study on TILs of the pleural effusion cytotoxcity with metastasis after the breast cancer operating Wu changping, Jiang Jingting, Deng Haifeng, et al Center Biological Diagnosis and Treatment of cancer Third Affiliated Hospital of Suzhou university.Jiangshu province ChangZhou 213003 Abstract Purpose: Analysising the cytotoxcity about tumour infilating lymphcoyte on the metastasis pleural effusion after the operation the cancer of the breast. Methods: With bitechnology and purification TILs and itself cells, to test the TILs cytoxcity to the itself cells of the tumour and K562 after on 7th 14th 21st and 28th. Results: The cytotoxicty of TILs difference were not significant statistically, It was the highest cytotoxcity in the third week. Conclusions: It will be acquired the reliable curative effection on malignant pleural effusion, this TILs had gained higher biology activity when the TILs on malignant pleural effusion were cultured in 21 days. Key words: breast cancer pleural effusion, malignat lymphocytes, tumour-infiltration cytotxicty 乳腺癌是危害妇女健康的主要恶性肿瘤 乳腺癌根治术后有10%~30%患者可能 发生局部和/或区域性复发 其中半数以上累及胸壁 从而引起胸腔积液 本室 曾采用流式细胞术分析了胸水中肿瘤浸润淋巴细胞 Tumor infiltration lymphocytes,TIL 的表型[1] 并用TIL治疗了36例癌性胸水 总有效率在 90%以上[2] 临床应用取得了满意的疗效 同时分析了28例乳腺癌术后胸膜转 移性癌性胸水对自身肿瘤细胞和 K562细胞毒活性 旨在更好地选择具有最佳 细胞毒活性的TIL应用于治疗该疾病引起的转移性胸水 1 材料