镍钛合金钛与钛铌涂层防止Ni2+析出的体内实验研究.DOCVIP

临床医学论文-镍钛合金钛与钛铌涂层防止Ni2+析出的体内实验研究 ?????????????作者：李永滨　孙明学　卢世璧 【关键词】? Ni2+, ??? 摘要：［目的］探讨镍钛合金(NiTi)表面金属钛(Ti)、钛铌合金(TiNb)涂层后对Ni2+析出的影响。［方法］成年犬18只，随机分为4组，NiTi组、Ti涂层组、TiNb涂层组各5只，空白组3只，分别于骶棘肌内和股骨内植入立方体试件和圆柱型试件各10枚，12个月后处死取材，用石墨炉原子吸收仪分别检测心、肝、脾，肺、肾、脑、骨、鼻咽粘膜、血液、肌肉、试件周围的骨组织和肌肉组织的Nit2+含量。［结果］非修饰的NiTi组试件周围的骨组织和肌肉组织的Ni2+含量与同组同种组织、异组同种组织的Ni2+含量有显著差异(P＜005)。［结论］本试验初步证明，NiTi表面金属钛、钛铌合金涂层起到了防止Ni2+析出的作用。 　　关键词：镍钛合金；钛；钛铌；Ni2+；析出；体内 　　Surface coating of Nitinol of titanium or titaniumniobium alloy to prevent separation of Ni2+ in vivo 　　Abstract：［Objective］To investigate the influence of modifying the surface of Nitinol by the coating of titanium or titaniumniobium alloy on separation of Nii2+ in vivo［Method］Eighteen adult dogs were divided randomly into 4 groups:Niti (5 dogs),Ti (5 dogs),TiNb (5 dogs),blank (3 dogs)Ten pieces of cubic implantation were put into sacrospinalis and 10 pieces of cylinder ones were put into femurs in each dog,the materials were obtained by sacrificing the dogs 12 months after the experiment,and the concentration of Nii2+ in hearts,livers,spleens,lungs,kidneys,brains,bones,mucous membrane of snasopharynx,bloods,muscles,tissues of bone near implantation and tissues of muscle near implantation were measured by atomic absorption spectrometry (GFAAS)［Result］Obvious difference (P＜005)of the concentration of Nii2+ were found between the tissues in the group of NiTi and the tissues in other groups［Conclusion］The experiment preliminarily proves that the coating of Ti or TiNb on the surface of Nitinol could stop separating Nii2+ from Nitinol 　　Key words：Nitinol; Titanium; TitaniumNiobium alloy; Ni2+;? Separation 　　镍钛合金(以下简称NiTi)具有其它金属类材料无可比拟的特性――超弹性和形状记忆性能，然而，镍的致敏性和可疑的致癌性使人们对其安全性产生疑虑。目前国内外主要解决办法是采用不同工艺、不同材料进行表面修饰，以提高其生物安全度。本实验以NiTi为基体，用等离子喷涂技术表面金属钛、钛铌合金涂层，以犬为实验动物，将不同形状试件植入犬的体内，1 a后处死取材，观察各脏器或组织Ni2+含量，以观察涂层对防止Ni2+析出效果。 　　1 材料和方法 　　11? 材料 　　材料植入前预处理，主要仪器和试剂、检测步骤、溶液制备和曲线绘制、统计学处理与上期《钛、钛铌涂层修饰对防止镍钛合金Ni2+析出的影响》基本相同。但本实验增加了柱形植入体，直径4 mm，长12 mm，基体和涂层成分和工艺与上述立方体相同。因本实验是检测有机物Ni2+